In Vitro Enhanced Production of Demethylwedelolactone

Abstract

Plants provide not only food, but also a wide range of chemical compounds, which perform variety of functions viz. pharmaceuticals, flavours, fragrance, colours and insecticides etc. These compounds belong to a group collectively known as secondary products or secondary metabolites. Eclipta alba is a well known medicinal plant and it is very popular in treatment of various liver ailments. Ex-tracts of this plant are used for the treatment of various hepatic disorders. In the present study an efficient and successful protocol for enhancement, accumulation and production of demethylwedelolactone from in vitro derived calli of Eclipta alba was established. Cul-tures were raised in different combinations - concentrations of plant growth regulators. The presence of 6-BA along with other combi-nation of growth regulators had a significant effect on the increase in concentration of demethylwedelolactone. Various epigenetic factors like temperature, pH, humidity etc also played crucial role in enhanced production of demethylwedelolactone.